Effect of metal ions and chemical agents on the activity of endoglucanase GH5 exploited from goats-rumen bacterial metagenomic DNA data

A gene coding for GH5 endoglucanase exploited from metagnomic DNA data of bacteria in Vietnamese goats’ rumen was modularity structure including a catalytic module, fibronectin-3 like module and an X module. The recombinant enzyme sucessfully expressed E. coli purified. To study the effect some metal ions chemicals on activity, this study, we used tools Swiss-Prot, ProFunc, COFACTOR prediction ligands interaction. obtained results indicated that most similar with had two conserved residues (Asp-190 và Asp-192) linked Mn2+ within radius ~ 3.5 Å center ion molecule contained disulphide bond. Experimental essessment (Ca2 +, Mn2 Mg2+, Ni2+, K+, Co2+, Cu2+, Zn2+, Fe3+) at final concentration 10 mM six common SDS (1%), urea (1 µM), 2-mercaptoethanol EDTA tween 80 (1mM), triton X-100 µM) showed only increased activity slightly times 40 Mn2+. has been identified as specific binding agent may increase stability GH5.